Lab 2 Introduction to Culturing & Aseptic Technique BIO250L
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Experiment 1- Agar Plate Preparation and Bacterial Inoculation
Table 1: Experiment 1 Colony Growth
Plate #
Condition/Surface
Growth (color, amount, shape, etc.)
1
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2
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3
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4
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5
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Post-Lab Questions
1. Which plate grew the most bacterial species (based on number of different colonies)? Was this a surprise? Why or why not?
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2. Was the control plate free of bacterial colonies? If yes, what does this mean for your experiment? If not, what could be the source of the contamination?
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3. Did the airborne contamination plate contain any growth? Were any of the colonies similar in appearance to those found on the other plates? How does this plate demonstrate the need for following aseptic technique when culturing bacteria?
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Experiment 2- Bacterial Transfer to a Stab Tube and an Agar Plate
Table 2: Initial Reserved Plate Colony Growth Observations
Colony
Plate Sample
Appearance (color, morphology, etc.)
1
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2
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3
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Table 3: Final Plate and Stab Tube Growth Observations
Sample
Form
Growth?
Same Appearance as Initial Plate?
Effective Transfer?
1
Plate
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Stab Tube
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2
Plate
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Stab Tube
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3
Plate
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Stab Tube
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Control
Plate
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Stab Tube
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Post-Lab Questions
1. Did the colonies that grew on the transfer plates retain the same appearance from the initial plate? Why or why not?
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2. Were the colonies uniform in appearance on the transfer? What could account for any differences seen?
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3. Did growth occur through the depth of the stab tube? If not, what could account for the lack of bacterial growth?
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4. Why was it important to include a control plate and stab tube in this experiment? Did they serve as positive or negative controls?
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Insert photo of your cultures after incubation with your name clearly visible in the background:
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